DNA fingerprinting evidence questioned

A DNA Fingerprinting Exercise for

"Hands on" laboratory exercises are excellent teaching tools because they have the potential to reach students with all types of learning styles. Unfortunately the large monetary investment required to obtain supplies and equipment and train instructors prevents many schools from providing hands-on learning experiences with DNA technology. Students in non-laboratory courses are even less likely...

DNA Fingerprinting and RFLP Analysis

Asbestos findings questioned.

The relationship between autocrine interferon (IFN) production and the expression of class I Major Histocompatibility Complex (MHC) membrane glycoproteins in vitro was investigated in a panel of murine transformed cells of nonhaemopoietic origin. The panel included 11 cell lines of H-2Kb haplotype derived from fibrosarcomas, carcinomas and melanoma, and from transformed fibroblasts. IFN activit...

Automated Image Analysis for Dna Fingerprinting

Methods for automated detection of DNA restriction fragments resolved on agarose fingerprinting gels are described. We present a mathematical model for the location and shape of the restriction fragments as a function of fragment size, with model parameters determined empirically from marker lanes containing molecular size standards. Automated identification of restriction fragments involves se...

The similarity index and DNA fingerprinting.

DNA-fingerprint similarity is being used increasingly to make inferences about levels of genetic variation within and between natural populations. It is shown that the similarity index--the average fraction of shared restriction fragments--provides upwardly biased estimates of population homozygosity but nearly unbiased estimates of the average identity-in-state for random pairs of individuals....